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Now available in pharmacies

Flora and microbiome

How to keep your vaginal microbiome healthy

Roxanne Sabbag

Co-founder and biomedical engineer, Santelle

7 min read

7 min read

You found yourself changing your panty liner three times today—not because of a leak, but because of a different smell you've noticed over the last few days.

You might be wondering if it's your new body wash, recent stress, or something else entirely.

It is probably not your body wash.

It is your vaginal microbiome sending you a signal. A natural ecosystem of bacteria lives in your vagina at all times, and it reacts to almost everything: your hormones, your cycle, your sleep, and even last night's cigarette.


What exactly is the vaginal microbiome?

You may have seen the word "flora" written on a prescription or a lab result without ever really being told what it means.

The vaginal microbiome is the collection of bacteria that naturally live in your vagina.

Unlike the gut, where having a wide variety of bacteria is a sign of good health, a healthy vagina works the opposite way: it is dominated by just one family of bacteria called lactobacilli (sometimes called Döderlein's bacilli).

These bacteria convert glycogen—a natural sugar found in the vaginal wall—into lactic acid.

This creates an acidic environment that is hostile to harmful germs and fungi like Candida albicans (the yeast responsible for thrush). Some strains even produce substances that directly target and neutralize these intruders.

So, this is not just about hygiene. It is an active, protective shield.


The five types of vaginal environments

If you have ever looked at a vaginal swab report, you might have seen "CST" followed by a Roman numeral and closed the tab because it looked like gibberish.

CST stands for community state types, which are the five main bacterial profiles researchers have identified in the vagina.

Four of these are dominated by a specific type of lactobacilli, while the fifth is a more diverse mix.

  • CST I, dominated by Lactobacillus crispatus: The most protective setup, featuring a low and stable pH level.

  • CST II, dominated by Lactobacillus gasseri: Less common, but highly stable and protective.

  • CST III, dominated by Lactobacillus iners: One of the most common types, but also the most unstable. This bacteria is found in healthy vaginas as well as during transition phases, such as after taking antibiotics.

  • CST IV, with no dominant lactobacilli: A diverse mix of different bacteria (like Gardnerella and Prevotella). This is the profile most commonly linked to an imbalance.

  • CST V, dominated by Lactobacillus jensenii: Less common and generally protective.

One important thing to remember: these categories are not set in stone. They change with your menstrual cycle, sexual activity, and pregnancy. Moving temporarily into a CST IV state does not automatically mean you have an infection. It is long-lasting changes with symptoms that actually matter.


Why your vaginal balance can get disrupted

A new birth control pill, the return of your period, or starting to smoke again after a break: any of these changes can be enough to disrupt your intimate balance.

Hormonal shifts

Estrogen indirectly feeds lactobacilli by helping the body produce glycogen.

This means any major hormonal shift—such as your monthly cycle, birth control, pregnancy, or menopause—changes the "food" supply available to these good bacteria.

Hygiene practices

The vagina is self-cleaning. Douching, scented products, and regular soaps—which are often too alkaline—disrupt the protective acidic environment rather than helping it.

Lifestyle

Long-term stress, poor sleep, and a diet high in sugar do not affect the vagina directly, but they do weaken your overall immune system and gut health, which in turn impacts your intimate health.

Smoking

This is one of the most overlooked factors.

Several studies link smoking to lower levels of protective lactobacilli and a higher risk of bacterial vaginosis (BV), likely because compounds from tobacco make their way into vaginal secretions.

Other factors also play a role: having multiple sexual partners without barrier protection, recent antibiotic use, or wearing damp synthetic clothing for too long.


Everyday tips for healthy intimate care

You might find yourself standing in the personal care aisle, trying to choose between different "intimate washes" with nearly identical packaging.

Here is what actually matters:

  • Wash no more than once a day using plain water or a soap-free wash with a skin-friendly pH.

  • Never douche: only wash the vulva (the outside), not the inside of the vagina.

  • Always wipe from front to back after using the toilet.

  • Choose breathable cotton underwear and avoid staying in wet swimwear for long periods.

  • Change period products regularly.

  • Avoid using scented products—like wipes, intimate deodorants, or sprays—near your intimate area.


The connection between your gut and your vagina

You have probably heard that eating yogurt or taking probiotic capsules can "help your intimate health," but wondered how a swallowed pill could possibly work all the way down there.

Here is how it happens. An oral probiotic capsule first travels through your digestive system. Your gut acts as a natural reservoir for lactobacilli, where certain strains temporarily settle.

From this reservoir, some of these bacteria naturally migrate to the vagina by traveling across the perineal area.

This path from gut to rectum to vagina is what researchers call the gut-vagina axis.

This pathway has been confirmed by clinical studies, particularly involving strains like Lactobacillus rhamnosus GR-1 and Lactobacillus reuteri RC-14.

Eating a diet rich in fiber and fermented foods supports this gut reservoir, while a high-sugar, low-fiber diet can deplete it.


Probiotics: Which ones actually work?

You might find yourself looking at a pharmacy shelf with a dozen different boxes, all promising to "restore your balance," with no clear explanation of how to choose between them.

There are two main options, each working differently. The table below compares them to help you decide which fits your needs.


Oral (Capsules)

Vaginal (Suppositories/Pessaries)

How it works

Systemic, via the gut-vagina axis

Local and direct

Time to take effect

One to two weeks

Faster acting

Extra benefits

Also supports gut health

Often used alongside or after antibiotic treatments

Some formulas, called synbiotics, combine probiotics (good bacteria) with prebiotics (compounds that feed and support those bacteria).

The strains with the most clinical research include Lactobacillus rhamnosus GR-1, Lactobacillus reuteri RC-14, Lactobacillus crispatus, Lactobacillus acidophilus, Lactobacillus plantarum, and Lactobacillus gasseri.

The evidence is promising for helping to prevent recurring bacterial vaginosis (BV), though results are more mixed when it comes to treating active yeast infections.

Two key things to remember: Probiotics are not a replacement for medical treatment if you have an active infection. They are meant to prevent issues or support recovery, not cure infections on their own.

It typically takes between one and four weeks of consistent use to notice a difference, so don't expect overnight results.


Puberty, pregnancy, menopause: An environment that changes with you

During puberty and your monthly cycle

Before puberty, the vaginal environment is very different from that of an adult. The rise of estrogen triggers the maturation of the tissue and helps lactobacilli move in and establish themselves.

Once established, this environment continues to shift every month. Period blood has a higher pH, which can temporarily disrupt this balance before it naturally resets a few days later.

During pregnancy and postpartum

During pregnancy, high estrogen levels often boost the dominance of Lactobacillus crispatus, which is linked to a lower risk of preterm birth.

On the other hand, a CST IV profile (where lactobacilli are low) during pregnancy is something healthcare providers will monitor closely.

After giving birth, the sudden drop in hormones and postpartum bleeding can temporarily weaken this protective barrier. It generally rebuilds itself gradually over the following weeks.

At menopause

A permanent drop in estrogen levels means less glycogen is produced to feed the lactobacilli.

With less food, the population of good bacteria drops, the pH level rises, and vaginal tissues can become drier.

This is a life stage where dryness, discomfort, and recurrent urinary tract infections (UTIs) become more common. Helpful options—such as vaginal moisturizers, local hormone therapy, or probiotics—can be discussed with your doctor to find what works best for you.


When should you see a doctor?

It is 3:00 AM, and you are typing your symptoms into a search engine hoping for a reassuring answer.

However, some symptoms require proper medical advice rather than a web search: a strong or unusual odor, grayish or greenish discharge, persistent itching, pain during sex, bleeding between periods, or pelvic pain accompanied by a fever.

Always get a proper diagnosis before starting any treatment.

Bacterial vaginosis, yeast infections, and certain sexually transmitted infections (STIs) can have very similar symptoms but require completely different treatments. A family doctor, midwife, or gynecologist can easily help you find the right solution.


FAQ: Questions you might hesitate to ask

Which probiotic should I choose for vaginal health?

It depends on what you need: are you looking to prevent infections, support an ongoing treatment, or navigate a specific stage of life?

Look for formulas that feature strains clinically tested for vaginal health, rather than just relying on marketing claims. A pharmacist can quickly guide you in the right direction.

Can I restore my vaginal balance without supplements?

Yes, to some extent. Good hygiene practices, wearing cotton underwear, and eating a balanced diet all help support your body's natural defenses.

However, if you have an ongoing or recurring imbalance, these habits alone may not be enough.

Does putting honey or yogurt in the vagina actually work?

No. These products do not contain the specific strains of bacteria needed for vaginal health. Furthermore, the sugars and texture of these kitchen ingredients can actually feed harmful germs and make things worse.

Healthcare professionals advise strongly against this practice.

How long does it take to restore vaginal balance?

According to clinical studies, it generally takes one to four weeks of consistent use to see a measurable difference. There is no reliable treatment that offers an instant fix.

Can the vaginal microbiome affect fertility?

This is an active area of medical research. Several studies suggest that an environment dominated by Lactobacillus crispatus provides a more supportive setting for embryo implantation, particularly during IVF treatments. However, this is just one of many factors involved in fertility.

Does my partner need to be treated if I have an imbalance?

Not typically for bacterial vaginosis (BV), as it is not classified as a sexually transmitted infection. However, for recurring yeast infections or STIs, the approach is different and should be discussed directly with your doctor.

Can stress really cause an intimate imbalance?

Indirectly, yes. Long-term stress can disrupt your hormone levels and your gut microbiome, which is the main source of beneficial vaginal lactobacilli. While it is not a direct cause, stress is a key factor that can affect your body's overall balance.

Frequently asked questions

Which probiotic should you choose for your vaginal flora?

It depends on your situation: prevention, alongside a treatment, or a particular stage of life. Check that the formula contains strains genuinely tested in clinical trials on the vaginal area, not just a marketing claim. A pharmacist can point you in the right direction in a few minutes.

Can you restore your flora without a supplement?

Yes, in part. Suitable hygiene, cotton underwear and a balanced diet already support the natural balance. With an established imbalance, or one that keeps returning, these measures alone are generally not enough.

Do honey or yoghurt in the vagina really work?

No. These products do not contain the specific strains suited to the vaginal ecosystem, and their sugar or texture can actually encourage unwanted organisms. No health professional recommends this practice.

How long does it take to restore your vaginal flora?

Allow one to four weeks of regular use to see a measurable effect, according to the available studies. There is no serious protocol promising an immediate result.

Can the vaginal microbiome influence fertility?

This is an active area of research. Flora dominated by Lactobacillus crispatus is associated, in several studies, with an environment more favourable to embryo implantation, particularly in assisted reproduction. It is, however, only one factor among others.

Should your partner be treated in the case of an imbalance?

Not systematically for bacterial vaginosis: it is not classed as a sexually transmitted infection as such. For a recurrent yeast infection or an STI, the question is different and is settled directly with your doctor.

Can stress really unbalance the vaginal flora?

Indirectly, yes. Chronic stress disrupts the hormonal axis and the gut microbiome, which itself feeds the reservoir of vaginal lactobacilli. It is not a direct link, but one factor among others weighing on the overall balance.

Written by

Roxanne Sabbag

Co-founder and biomedical engineer, Santelle

Roxanne co-founded Santelle and is a biomedical engineer specialising in vaginal health.


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Ten minutes to understand your body better.

Ten minutes to understand your body better.

The Santelle kit is a self-test for monitoring and information. It does not replace a consultation or a medical diagnosis. If your symptoms persist or seem unusual, speak to a healthcare professional.

The Santelle kit is a self-test for monitoring and information. It does not replace a consultation or a medical diagnosis. If your symptoms persist or seem unusual, speak to a healthcare professional.